ABSTRACT
BACKGROUND: Congenital rubella syndrome (CRS) case identification is challenging in older children since laboratory markers of congenital rubella virus (RUBV) infection do not persist beyond age 12 months. METHODS: We enrolled children with CRS born between 1998 and 2003 and compared their immune responses to RUBV with those of their mothers and a group of similarly aged children without CRS. Demographic data and sera were collected. Sera were tested for anti-RUBV immunoglobulin G (IgG), IgG avidity, and IgG response to the 3 viral structural proteins (E1, E2, and C), reflected by immunoblot fluorescent signals. RESULTS: We enrolled 32 children with CRS, 31 mothers, and 62 children without CRS. The immunoblot signal strength to C and the ratio of the C signal to the RUBV-specific IgG concentration were higher (P < .029 for both) and the ratio of the E1 signal to the RUBV-specific IgG concentration lower (P = .001) in children with CRS, compared with their mothers. Compared with children without CRS, children with CRS had more RUBV-specific IgG (P < .001), a stronger C signal (P < .001), and a stronger E2 signal (P ≤ .001). Two classification rules for children with versus children without CRS gave 100% specificity with >65% sensitivity. CONCLUSIONS: This study was the first to establish classification rules for identifying CRS in school-aged children, using laboratory biomarkers. These biomarkers should allow improved burden of disease estimates and monitoring of CRS control programs. Published by Oxford University Press on behalf of the Infectious Diseases Society of America 2014. This work is written by (a) US Government employee(s) and is in the public domain in the US.
Subject(s)
Schools , Students , Rubella Syndrome, Congenital/diagnosis , Biomarkers/blood , Adolescent , Antibodies, Viral , Antibody AffinityABSTRACT
BACKGROUND: In order to shorten the programming process of cochlear implanted (CI) children it is possible to establish the T level (threshold level) as being 10% of the C level (most comfortable level). AIM: To compare the results of speech perception tests using the map of the real thresholds levels and the map corresponding to 10% of the C level. MRTHODS: A list of monosyllables and sentences were used with 30 CI individuals in two programming situations. RESULTS: no difference was found between the results in the speech tests in the two programming situations. CONCLUSION: The use of the map with the T level corresponding to 10% of the C level can be an option to program young children, especially for those who do not respond to the investigation of the real T levels.
Tema: para abreviar o processo de mapeamento das crianças usuárias de implante coclear (IC) é possível estabelecer o nível T (threshold level) como sendo 10% do nível C (mostconfortable level). Objetivo: comparar o resultado dos testes de percepção de fala, utilizando limiares reais e com correspondentes a 10% do nível C. Método: lista de monossílabos esentenças foram aplicadas em trinta usuários de IC nas duas situações de programação. Resultados: não existiram diferenças nos testes de fala nas duas situações de programação. Conclusão: a utilização do mapa contendo nível T igual a 10% do nível C seria uma opçãopara as crianças que ainda não conseguem realizar a pesquisa do nível T real..